Sperm capacitation by density gradient centrifugation

Last Update: 09/10/2018

Density gradient centrifugation allows us to separate a sperm sample depending on the spermatozoa’s ability to go through several media, each with a different density.

Sperm with the best quality and motility will be able to reach the bottom of the tube, that is to say, to go through all the media in spite of their increasing density level. These sperms will be the ones to use for the fertility treatment of choice.

Experts typically use two or three density gradients. The most common gradients are 45%, 80% and 90%.

Read the full article on: ( 57).
By Andrea Rodrigo BSc, MSc (embryologist), Luis Gijón Tévar BSc, MSc, PhD (embryologist) and Mark P. Trolice MD, FACOG, FACS, FACE (reproductive endocrinologist).
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