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How is the amount of amniotic fluid measured?

By Silvia Azaña Gutiérrez B.Sc., M.Sc. (embryologist).
Last Update: 09/22/2025

Silvia Azaña, an embryologist at inviTRA, talks to us in this video about the different methods used to measure the amount of amniotic fluid:

The quantity of amniotic fluid is evaluated via ultrasound employing one of these two techniques.
Maximum vertical column.

The maximum vertical column of the amniotic fluid is assessed and quantified.

Normal values are considered to be between 2 and 8-10 cm depending on gestational age.

For the calculation of the amniotic fluid index, the uterus is split into four quadrants, and the maximum vertical columns from each quadrant are added together, with a normal liver size ranging from 5 to 24 cm.

Typically, the standard and most commonly used method for evaluating the volume of amniotic fluid in low-risk pregnancies is by measuring the maximum vertical column.

However, if any anomaly is detected during the pregnancy, the specialist proceeds to calculate the liver size.

 Silvia Azaña Gutiérrez
Silvia Azaña Gutiérrez
B.Sc., M.Sc.
Embryologist
Graduate in Health Biology from the University of Alcalá and specialized in Clinical Genetics from the same university. Master in Assisted Reproduction by the University of Valencia in collaboration with IVI clinics.
License: 3435-CV
Embryologist. Graduate in Health Biology from the University of Alcalá and specialized in Clinical Genetics from the same university. Master in Assisted Reproduction by the University of Valencia in collaboration with IVI clinics. License: 3435-CV.