Male hormone analysis: what should the normal hormone levels be?

By (embryologist), (embryologist), (gynecologist), (biochemist) and (fertility counselor).
Last Update: 09/05/2022

Male sex hormones play a major role in fertility, since they are directly associated with the sperm production process, which is called spermatogenesis. The most important hormones involved in this function are testosterone, dihydrotestosterone, LH, and FSH.

Hormone analysis in men is carried out through a blood test. If the results obtained show altered values of some of these hormones, it could the reason for the difficulty experienced in becoming a father.

Male fertility tests

Achieving a pregnancy is not always an easy task. If it has not happened after trying to conceive for a year, couples are strongly recommended to visit a fertility specialist to see if there is some kind of infertility issue that is preventing pregnancy.

Infertility specialists typically ask new patients to undergo a complete fertility evaluation to check fertility in both the man and the woman, since both play a part in the TTC journey, and any abnormality can be the cause of infertility totally or partially.

To check male fertility, the simplest, most informative test is a semen analysis report. It involves examining a semen sample to look for the presence of sperm cells, and see if they are normal in terms of motility, concentration, morphology, etc.

Assisted procreation, as any other medical treatment, requires that you rely on the professionalism of the doctors and staff of the clinic you choose. Obviously, each clinic is different. Get now your Fertility Report, which will select several clinics for you out of the pool of clinics that meet our strict quality criteria. Moreover, it will offer you a comparison between the fees and conditions each clinic offers in order for you to make a well informed choice.

In case that severe abnormalities are found through a semen analysis, such as azoospermia or cryptozoospermia, your doctor may ask you to do a blood test to check your hormone levels and try to find the cause of the poor sperm quality. Sometimes, sperm quality can be improveds with a hormone treatment.

According to Valeria Sotelo, MD:

The first fertility test done in males is a semen analysis. It is used to evaluate the sperm count, motility, and morphology. If the first semen analysis is normal, no other tests are required. But, if the results are abnormal, the man has to repeat it after 15 days.

Function of male sex hormones

Regulation of male sex hormones starts in the hypothalamus, a crucial part of the brain. Its function is to trigger the release of GnRH hormone, responsible for stimulating the pituitary gland to produce FSH and LH.

FSH and LH

The functions of both FSH and LH are interrelated between each other, since both affect the testicular function of males.

On the one hand, FSH regulates spermatogenesis (formation of new spermatozoa) in the seminiferous tubules of the testis. It affects the Sertoli cells, which are responsible for protecting and nourishing the cells that lead to sperm formation (spermatogonia). Moreover, FSH promotes the production of the testicular proteins that bind androgens (ABP).

On the other hand, LH triggers the production of testosterone by stimulating Leydig cells. Moreover, thanks to the action of ABP, testosterone can influence the testis and play its part on spermatogenesis.

Testosterone and dihydrotestosterone

Testosterone is the main male sex hormone. It is produced in the testicles due to the action of LH. It is also produced by the adrenal glands in both men and women, as well as by the ovaries in women, although in a small quantities.

5α-dihydrotestosterone (5α-DHT) derives from testosterone, that is, it is generated out of testosterone thanks to the action of 5α-reductases enzymes.

Both hormones are involved in the development of male sex characteristics, including body mass building, as well as facial and body hair growth.

If you would like to continue reading about this male sex hormone then please take a look at this the following article: What is the function of testosterone?


As it happens with FSH and LH, prolactin is also produced by the pituitary gland, and is involved in male fertility to a large extent.

Prolactin is necessary for the well functioning of the male reproductive system, and affects the Leydig cells and testosterone production directly. As such, if the male has high levels of prolactin, the testosterone levels will be affected.

Male hormone reference ranges

Abnormal variations in the levels of the above mentioned hormones can affect spermatogenesis, either directly or indirectly. The following are the main hormones checked in males to find potential abnormalities:

Testosterone and dihydrotestosterone

Within the organism, testosterone can be free or linked to proteins. These are the normal testosterone levels in adult males:

  • Free testosterone (bioavailable, not bound to proteins): 90-300 pg/mg.
  • Total testosterone (free + bound to proteins): 270-1070 ng dl.

If the levels of testosterone are low, it can indicate there exists some sort of abnormality that is affecting sperm production, like damage to the testes. This can cause male infertility.

Certain syndrome can cause  low testosterone levels, including Klinefelter syndrome or Kallmann syndrome.

If testosterone levels are high, it could be due to a testicular tumor or androgen resistance amongst other potential causes. Certain substances or drugs can also increase testosterone levels.

Finally, normal levels of 5α-DHT are 30-85 ng/dl. Insufficient levels of 5α-reductases enzymes can prevent the synthesis of 5α-DH, causing pseudohermaphroditism (a person that has the secondary sex characteristics of a female but is a man from the genetic viewpoint).

FSH and LH

If your doctor suspects that your male sexual characteristics are abnormal, or you have erectile dysfunction, low libido or infertility issues, he may request a blood test to examine your FSH and LH levels. The reference rangess are:

  • FSH: 1.0-12.0 mIU/ml.
  • LH: 2.0-12.0 mIU/ml.

Elevated levels of FSH and/or LH may indicate primary testicular failure (PTF), which can be the result of a wide range of indirect causes such as infection, trauma, chemotherapy etc.

Conversely, levels are too low indicate hypothalamic dysfunction or abnormalities in the pituitary gland.


Normal prolactin levels should be between 2.5 and 17 ng/ml (53-360 mIU/l). If they are above this reference range, it can indicate any of the following abnormalities:

  • Low testosterone levels
  • Sexual dysfunction
  • Gynecomastia (abnormal growth of mammary glands)
  • Infertility
  • Hypothyroidism
  • Pituitary tumor

Prolactin can also be analyzed for clinical follow-up of men who have low testosterone concentrations.

FAQs from users

My hormone levels are normal but I have azoospermia. Is it normal?

By Rebeca Reus BSc, MSc (embryologist).

It can be that the hormonal values are normal, but that there is some other problem affecting sperm production or the exit of the spermatozoa to the exterior.

In the latter case, this alteration is called obstructive azoospermia and, sometimes, sperm can be obtained by means of a testicular biopsy.

However, it should be borne in mind that it is not always possible to find the cause of sterility in both males and females.

What treatment options do I have for abnormal testosterone levels?

By Rebeca Reus BSc, MSc (embryologist).

Artificial testosterone can be administered through different routes:

  • Intramuscular injection: there exist different types of synthetic testosterone that can be administered intramuscularly. Propionate remains for a reduced time period in the body, so it has to be administered every 2-3 days. Testosterone enanthate and cypionate can be injected every two weeks (dose of 200 mg). Conversely, testosterone undecanoate can be administered every 12-15 weeks (1000 mg).
  • Transfermal (patch): it can be administered in the form of a gel or cream daily, in the form of patches or implant.
  • Oral (pills): this is the least common of all routes, since it is detrimental for the liver (hepatic toxicity).

Even though artificial testosterone can have many benefits, like muscle development or secondary male characteristics, one should keep in mind that, as with any other medication, it can have some side effects. Always follow your doctor's instructions before taking this type of medication.

What effects do hormones have on male fertility?

By Marta Barranquero Gómez B.Sc., M.Sc. (embryologist).

Generally, hormonal analysis in men to study their fertility consists of determining the levels of testosterone, FSH (Follicle Stimulating Hormone) and LH (Luteinizing Hormone).

FSH is involved in spermatogenesis, that is, in the formation of spermatozoa in the seminiferous tubules.

On the other hand, LH acts on the Leydig cells and secretes testosterone, the male sex hormone par excellence.

Finally, testosterone is involved in the development of male sexual characteristics. Thus, testosterone is responsible for the growth of muscle mass, beards and body hair.

In addition to an hormone test, there are other tests a man can undergo to learn more about his fertility. If you are interested in delving deeper into them, we recommend that you have a look at this guide: Male Fertility Testing – How Do You Know if You Are Infertile?

The most common, informative of all male fertility tests is, as we explained above, the semen analysis (SE). We recommend that you get more info on this test here: What Is a Semen Analysis Report? – Purpose, Preparation & Cost.

We make a great effort to provide you with the highest quality information.

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Reproducción Asistida ORG. Video: ¿Qué pruebas se realizan en el hombre y en qué consisten? (Male fertility tests and their usefulness), by Valeria Sotelo, MD, Apr 7, 2015. [See original video in Spanish].

FAQs from users: 'My hormone levels are normal but I have azoospermia. Is it normal?', 'What treatment options do I have for abnormal testosterone levels?' and 'What effects do hormones have on male fertility?'.

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Authors and contributors

 Marta Barranquero Gómez
Marta Barranquero Gómez
B.Sc., M.Sc.
Graduated in Biochemistry and Biomedical Sciences by the University of Valencia (UV) and specialized in Assisted Reproduction by the University of Alcalá de Henares (UAH) in collaboration with Ginefiv and in Clinical Genetics by the University of Alcalá de Henares (UAH). More information about Marta Barranquero Gómez
License: 3316-CV
 Rebeca Reus
Rebeca Reus
BSc, MSc
Degree in Human Biology (Biochemistry) from the Pompeu Fabra University (UPF). Official Master's Degree in Clinical Analysis Laboratory from the UPF and Master’s Degree about the Theoretical Basis and Laboratory Procedures in Assisted Reproduction from the University of Valencia (UV). More information about Rebeca Reus
 Valeria Sotelo
Valeria Sotelo
M.D., M.Sc.
Bachelor's Degree in Medicine from the University of Buenos Aires. Specialist in Gynecology & Obstetrics. Master's Degree in Video-laparoscopic Surgery, and Certificate of Specialist in Gynecology. Associate Member of the Spanish Fertility Society (SEF) and the Spanish Society of Gynecology & Obstetrics. More than 10 years of experience in the field of Reproductive Medicine. More information about Valeria Sotelo
License: 030309166
Adapted into english by:
 Michelle Lorraine Embleton
Michelle Lorraine Embleton
B.Sc. Ph.D.
PhD in Biochemistry, University of Bristol, UK, specialising in DNA : protein intereactions. BSc honours degree in Molecular Biology, Univerisity of Bristol. Translation and editing of scientific and medical literature.
More information about Michelle Lorraine Embleton
 Sandra Fernández
Sandra Fernández
B.A., M.A.
Fertility Counselor
Bachelor of Arts in Translation and Interpreting (English, Spanish, Catalan, German) from the University of Valencia (UV) and Heriot-Watt University, Riccarton Campus (Edinburgh, UK). Postgraduate Course in Legal Translation from the University of Valencia. Specialist in Medical Translation, with several years of experience in the field of Assisted Reproduction. More information about Sandra Fernández

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