In this video, Silvia Azaña, an embryologist at inviTRA, explains the classification of vacuolated sperm:
One of the most commonly used criteria for sperm classification when performing morphological evaluation at high magnification is the following.
- Grade I: Absence of vacuoles.
- Grade II: Two small vacuoles maximum.
- Grade III: More than two small vacuoles or at least one large vacuole.
- Grade IV: Large vacuoles and associated with other abnormalities.In this way, as the degree increases, the morphological alterations of the spermatzoa increase.
Read the full article on: What are vacuoles in spermatozoa and do they have implications? ( 35).

Silvia Azaña Gutiérrez
B.Sc., M.Sc.
Graduate in Health Biology from the University of Alcalá and specialized in Clinical Genetics from the same university. Master in Assisted Reproduction by the University of Valencia in collaboration with IVI clinics.
License: 3435-CV