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How are vacuolated sperm classified?

By Silvia Azaña Gutiérrez B.Sc., M.Sc. (embryologist).
Last Update: 01/23/2026

In this video, Silvia Azaña, an embryologist at inviTRA, explains the classification of vacuolated sperm:

One of the most commonly used criteria for sperm classification when performing morphological evaluation at high magnification is the following.

- Grade I: Absence of vacuoles.
- Grade II: Two small vacuoles maximum.
- Grade III: More than two small vacuoles or at least one large vacuole.
- Grade IV: Large vacuoles and associated with other abnormalities.

In this way, as the degree increases, the morphological alterations of the spermatzoa increase.

 Silvia Azaña Gutiérrez
Silvia Azaña Gutiérrez
B.Sc., M.Sc.
Embryologist
Graduate in Health Biology from the University of Alcalá and specialized in Clinical Genetics from the same university. Master in Assisted Reproduction by the University of Valencia in collaboration with IVI clinics.
License: 3435-CV
Embryologist. Graduate in Health Biology from the University of Alcalá and specialized in Clinical Genetics from the same university. Master in Assisted Reproduction by the University of Valencia in collaboration with IVI clinics. License: 3435-CV.