The technique of cryopreservation of the ovarian cortex is based on the removal of the cortex to cryopreserve it in small pieces. The success of this technique is due to the following:
- The small primordial and primary follicles containing immature eggs are preserved at low temperature: immature eggs have a higher survival rate to freezing than mature eggs found in mature eggs. This is because immature eggs are small and have not yet developed structures, such as the zona pellucida and cortical granules, which are susceptible to damage at freezing temperatures.
- The cortex is divided into small ovarian strips: the division of the cortex into small portions of 1.5 cm by 0.5 cm. The division into small strips causes substances used to protect the ovarian tissue from freezing damage (also called cryoprotectants) to penetrate the ovarian tissue more evenly.
By Blanca Paraíso MD, PhD, MSc (gynecologist), Cristina Mestre Ferrer BSc, MSc (embryologist), Juan Antonio García Velasco MD, PhD (gynecologist), Laura Parra Villar (embryologin), Dr. Marita Espejo Catena BSc, MSC, PhD (gynecologist) and Romina Packan (invitra staff).
Last Update: 04/15/2020