A sperm wash or isolate procedure is used depending on semen factors at the time of the egg retrieval. Typically, if a semen sample is within the normal range, an isolate procedure is used. Alternatively, if a semen sample demonstrated out of range parameters, a washing procedure is used. In men who have a known male factor during the initial infertility evaluation, our practice is to use the wash method, while the isolate procedure is used on men without a male factor. Both procedures are used for sperm to achieve capacitation.
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