A sperm wash or isolating procedure is used depending on semen factors at the time of the egg retrievall, in folliclar puncture.
Typically, if a semen sample is within the normal range, a gradient density isolation procedure is used. Alternatively, if a semen sample shows values out of the range of normal parameters, a washing procedure is used. If, in the initial infertlity evaulation a problem is dected in the male, our practice is to use the wash method, while the gradient density isolate procedure is used on men with normal sperm. Both procedures are result in sperm capacitation.