Yes, in sperm FISH defined and not superimposed contours must be evaluated and all hybridization signals must be perfectly located inside the nucleus.
In addition, in cases where more than one signal is detected for each chromosome analysed, all signals must be of equal intensity.
Read the full article on: Fluorescent in situ hybridization FISH: Alterations in Sperm Cells ( 82).
Marta Barranquero Gómez
B.Sc., M.Sc.
Graduated in Biochemistry and Biomedical Sciences by the University of Valencia (UV) and specialized in Assisted Reproduction by the University of Alcalá de Henares (UAH) in collaboration with Ginefiv and in Clinical Genetics by the University of Alcalá de Henares (UAH).
License: 3316-CV
