At the time of intracytoplasmic sperm injection, there are 4 types of breakage depending on how the microinjection pipette is introduced into the egg. Here are them:
- Breakage using pressure without vibration: the plasma membrane breaks without making pressure or forming the cone. It is associated with poor quality eggs.
- Breakage using pressure A1: a cone is formed by the pressure applied until the plasma membrane breaks.
- Breakage using aspiration A2: if there has not been a breakage by pressure once inside the egg, cytoplasm is aspirated slightly until a jump indicates that the membrane has ruptured.
- Breakage using aspiration A3: is the same as above, but rupture occurs when cytoplasm is aspirated beyond the zona pellucida.
By Gorka Barrenetxea Ziarrusta M.D., Ph.D. (gynecologist), Dr. José León Tovar M.D., M.Sc. (gynecologist), José María Sánchez Jordán M.D. (gynecologist), María de Las Heras Martínez B.Sc., M.Sc. (clinical embryologist), Marta Barranquero Gómez B.Sc., M.Sc. (embryologist), Zaira Salvador B.Sc., M.Sc. (embryologist) and Michelle Lorraine Embleton B.Sc. Ph.D. (biochemist).
Last Update: 02/27/2019