At the time of intracytoplasmic sperm injection, there are 4 types of breakage depending on how the microinjection pipette is introduced into the egg. Here are them:
- Breakage using pressure without vibration: the plasma membrane breaks without making pressure or forming the cone. It is associated with poor quality eggs.
- Breakage using pressure A1: a cone is formed by the pressure applied until the plasma membrane breaks.
- Breakage using aspiration A2: if there has not been a breakage by pressure once inside the egg, cytoplasm is aspirated slightly until a jump indicates that the membrane has ruptured.
- Breakage using aspiration A3: is the same as above, but rupture occurs when cytoplasm is aspirated beyond the zona pellucida.
Read the full article on: The ICSI Process – How Does It Work Step by Step? ( 83).
By Gorka Barrenetxea Ziarrusta M.D., Ph.D. (gynecologist), María de Las Heras Martínez B.Sc., M.Sc. (clinical embryologist), Zaira Salvador B.Sc., M.Sc. (embryologist) and Marie Tusseau (invitra staff).
Last Update: 02/27/2019
