The number of sperm per milliliter of semen can be easily measured using a counting chamber. Typically, the Makler chamber is used, a device that has a grid with 10 rows and 10 columns, i.e. 100 squares in total. The number of spermatozoa in any line of 10 squares, whether row, column or diagonal, will indicate the sperm count in millions of spermatozoa per milliliter.
When this value is multiplied by the volume of ejaculate obtained by the male, the total sperm concentration will be known.