In order for sperm viability not to be compromised during the sperm freezing process, the sample must be covered with cryoprotectants. Samples are stored in liquid nitrogen at -196 °C until they are used in a fertility treatment, either IVF or ICSI.
Process of sperm freezing
By Antonio Alcaide Raya BSc, MSc, PhD (senior embryologist), Juan Antonio García Velasco MD, PhD (gynecologist), Rebeca Reus BSc, MSc (embryologist), Dr. Rocío Núñez Calonge PhD, MSc (senior embryologist) and Sandra Fernández BA, MA (fertility counselor).
Last Update: 01/18/2019