There are many protocols for slow egg freezing. Next, we will describe what a slow freezing ramp would look like in general:
- From room temperature to about -7 °C at a rate of about 2 °C/min.
- Maintain 5 minutes at -7 °C to make a manual seeding and induce the formation of a crystal in the solution.
- A cooling speed of about 0.3 °C/min is programmed until a temperature between -40 and -70 °C is reached.
- Finally, the ova are introduced directly into liquid nitrogen to be kept frozen at -196 °C.
Read the full article on: What’s the difference between freezing and vitrification of eggs? ( 60).
By Andrea Rodrigo B.Sc., M.Sc. (embryologist), Felipe Gallego Terris B.Sc, M.Sc (embriologist), Gustavo Daniel Carti M.D. (gynecologist), Paloma de la Fuente Vaquero M.D., Ph.D., M.Sc. (gynecologist), Sara Salgado B.Sc., M.Sc. (embryologist), Zaira Salvador B.Sc., M.Sc. (embryologist), Michelle Lorraine Embleton B.Sc. Ph.D. (biochemist) and Romina Packan (invitra staff).
Last Update: 10/24/2019