Slow egg freezing

There are many protocols for slow egg freezing. Next, we will describe what a slow freezing ramp would look like in general:

• From room temperature to about -7 °C at a rate of about 2 °C/min.
• Maintain 5 minutes at -7 °C to make a manual seeding and induce the formation of a crystal in the solution.
• A cooling speed of about 0.3 °C/min is programmed until a temperature between -40 and -70 °C is reached.
• Finally, the ova are introduced directly into liquid nitrogen to be kept frozen at -196 °C.