Before doing ICSI, it is necessary to prepare the culture plate with the oocyte to be fertilized and a sample with the capacitated sperm.
First, the embryologist has to select the highest quality sperm and then immobilize it with a pipette to the tail. Once immobilized, it is aspirated with the microinjection pipette.
On the other hand, the ovum is held with the holding pipette and must also be properly oriented so that its polar body is at the top or bottom.
Read the full article on: How the ISCI procedure is performed – the treatment step by step ( 85).
By Gorka Barrenetxea Ziarrusta M.D., Ph.D. (gynecologist), Dr. José León Tovar M.D., M.Sc. (gynecologist), José María Sánchez Jordán M.D. (gynecologist), María de Las Heras Martínez B.Sc., M.Sc. (clinical embryologist), Marta Barranquero Gómez B.Sc., M.Sc. (embryologist), Zaira Salvador B.Sc., M.Sc. (embryologist) and Michelle Lorraine Embleton B.Sc. Ph.D. (biochemist).
Last Update: 02/27/2019
