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Hypoosmotic test for the study of sperm vitality
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Hypoosmotic test for the study of sperm vitality

The hypoosmotic test is a test to assess sperm vitality, although it is a technique little used today. It involves putting the sperm in a hypotonic medium, which causes an osmotic imbalance between the extracellular and intracellular medium.

In this way, it is possible to distinguish between:

  • Live spermatozoa: they react to the change of medium, causing the tail to curl.
  • Dead spermatozides: they do not react to the hypotonic medium, so they show the smooth thing.

To obtain a reliable result of the vitality test, it is necessary to study at least 200 sperm. When the percentage of live sperm is greater than 58%, it will be said that the sperm vitality is correct. However, if the percentage of live sperm is less than 58%, the seminal sample will be diagnosed as necrozoospermia.

Read the full article on: ( 39).
By (embryologist), (embryologist), (embryologist), (gynecologist) and (psychologist).
Last Update: 02/28/2022