The ovarian stimulation that occurs in an artificial insemination treatment is very mild and lasts 8-14 days. During this period it is necessary to make a control through estradiol analysis and transvaginal ultrasounds in order to verify that the hormonal medication is working as expected.
The number and size of follicles in the ovaries is observed and ovulation is programmed when there are between 1 and 3 follicles of about 18 mm. Approximately 34-36 hours after ovulation is induced with the hCG injection, artificial insemination takes place.
If more follicles have developed with the stimulation, it would be necessary to cancel the AI, as there would be a risk of causing a multiple pregnancy.