For egg devitrification, it is necessary to follow a protocol so that the integrity and survival of the eggs are not affected.
First, the Cryotop is removed from the liquid nitrogen tank, the lid is removed and the Cryotop is placed directly into a 37°C devitrification medium. Here the eggs must remain for 1 minute, during which time they must be detached from the Cryotop.
The eggs must then be passed through different culture media with a decreasing concentration of cryoprotectants so that they can remove these substances and rehydrate properly.
Finally, it is necessary to check under the microscope whether the eggs are alive and can be fertilized without problems.