To perform ICSI, the first steps involved are:
- Ovarian stimulation: consists of administering hormonal medication to the woman so that more eggs can be obtained.
- Follicular puncture: is the process by which the eggs are extracted.
- Decumulation: once the eggs have been obtained, the cells surrounding them are removed to facilitate micromanipulation and to better assess their maturity.
- Semen preparation: consists of capacitating the sample and separating the best spermatozoa for microinjection.
At this point, it is important to mention that, if sperm cannot be obtained from the male ejaculate, they can be obtained by testicular biopsy or epididymal aspiration.
By Aitziber Domingo Bilbao B.Sc., M.Sc. (embryologist), Gustavo Daniel Carti M.D. (gynecologist), Joel G. Brasch M.D. (gynecologist), Marta Barranquero Gómez B.Sc., M.Sc. (embryologist), Miguel Dolz Arroyo M.D., Ph.D. (gynecologist), Rebeca Reus BSc, MSc (embryologist), Silvia Azaña Gutiérrez B.Sc., M.Sc. (embryologist), Susana Cortés Gallego B.Sc., Ph.D. (senior embryologist), Zaira Salvador B.Sc., M.Sc. (embryologist) and Michelle Lorraine Embleton B.Sc. Ph.D. (biochemist).
Last Update: 11/30/2021