Egg vitrification process

The egg cryopreservation process is carried out using the vitrification technique, which uses ultra-fast cooling speeds. However, in order to protect the eggs from damage during the process, it is necessary to use cryoprotective substances, which replace the intracellular water in the eggs and prevent the formation of ice crystals.

Therefore, before immersing the eggs in liquid nitrogen, they must be placed in wells containing the cryoprotective substances. After this process, the eggs must be carefully deposited on the support used for vitrification (the most common today is the Cryotop of the Kitazato brand).