Pregnancy symptoms but a negative test, what else could it be?

By (gynecologist), (embryologist), (gynecologist), (embryologist), (psychologist) and (biochemist).
Last Update: 07/18/2022

It's common to have doubts about the reliability of a pregnancy test, especially if the woman shows signs of being pregnant but the result is negative.

In this situation, it´s normal for a woman to wonder if it´s possible for the test to show a negative result even though she actually is pregnant. This type of result is known as false negative and yes, it´s possible for it to occur in pregnancy tests

Beta-hCG hormone

Pregnancy tests, tested both in urine or in blood, measure the human chorionic gonadotropin (hCG) hormone. This hormone is secreted by the embryo in large quantities, so it is characteristic of pregnancy,. Typically, hCG levels double every 48-72 hours in the first trimester of pregnancy. This increase in hCG levels in the mother's body is responsible for the usual pregnancy symptoms.

Among the functions of hCG is the prevention of disintegration of the ovarian corpus luteum so that it continues to produce progesterone. Furthermore, hCG is involved in immune tolerance during pregnancy, protecting the early fetus from immune rejection. However, hCG secretion begins to progressively decrease at about 3 months of gestation.

If you want to know more about the pregnancy hormone, you can click here: What Are Normal hCG Hormone Levels during Pregnancy?

False negative pregnancy test

The blood pregnancy test has a higher sensitivity than the urine pregnancy test. Since the blood test can detect lower levels of hCG hormone, the likelihood of a false negative is lower than with the urine test.

Causes of a false negative

The reasons for a false negative pregnancy test are:

  • The test has been done too early: if at least 8-12 days have not passed since ovulation or there is no delay in menstruation, it´s quite possible that the hormone levels are not high enough to be detected by the test.
  • The test has not been done with the first urine of the day: if a urine pregnancy test is done, it´s important to do it with the first urine of the day because this is when it will contain the highest concentration of the hCG hormone.
  • The woman is taking or has taken any medication that may affect the result.
  • The test chosen is of low sensitivity, for example, it detects hCG levels above 20 mIU/ml. This means that the test will not be able to detect pregnancy at an early stage.
  • Early pregnancy loss.
  • The pregnancy test is expired or damaged.

In case any of these situations occur, the best way to check whether or not there is indeed a pregnancy is to repeat the test 2-3 days later to confirm the result.

Why do I feel pregnant if I am not?

As mentioned above, a woman can feel pregnant and have negative pregnancy tests. However, if symptoms of pregnancy persist and doubt remains, the woman should consult her physician.

The doctor will order an hCG blood test to confirm whether or not there is a pregnancy and perform a transvaginal ultrasound to directly observe the inside of the uterus to look for the gestational sac and embryo. Furthermore, the specialist should also perform a physical examination and check the size of the uterus.

Psychological pregnancy

If the doctor rules out pregnancy, the woman could be facing a psychological pregnancy. The causes of this type of pregnancy can be diverse, for example, if the woman has a very strong desire to become a mother or a terrible fear of pregnancy.

Symptoms of psychological pregnancy include:

  • Lack of menstruation (amenorrhea).
  • Increase in breast size, aureole and breast pigmentation.
  • Darkening of the skin.
  • Increase in the size of the abdomen.
  • Significant weight gain, more than in a normal pregnancy, and strong increase in appetite.
  • Appearance of nausea and gastric problems such as heartburn.
  • Presence of sleep disorders.
  • Cravings.

The physician may request additional tests to verify that these symptoms are not due to some other disorder and to confirm that it is indeed a psychological pregnancy. If this diagnosis is confirmed, the woman will need to start psychological therapy. The specialized professional may prescribe medication, as well as working at home with the couple to overcome it.

If you want to know more about psychological pregnancy, you can access this article: What Is a False Pregnancy or Pseudocyesis?

Other causes of menstrual delay

Once the possibility of pregnancy has been ruled out, delays in the menstrual period indicate the presence of irregularities in the menstrual cycle. These irregularities can occur in all women at some point in their lives.

The reasons for a menstrual delay can be varied:

Polycystic Ovaries
women with polycystic ovary syndrome may have infrequent or prolonged menstrual periods, have excessive levels of male hormone (androgen), and their most frequent symptoms are hirsutism (excess hair), centripetal obesity, and menstrual irregularity. It is one of the most common disorders in women and its prevalence is estimated between 5 and 20%.
Change of habits
changes en daily routine can influence the hormonal cycle. Women who make drastic dietary changes, severe exercise practice or a major emotional life change may experience cycles. Therefore, it is important to maintain good, balanced dietary habits.
is one of the main enemies because it increases the production of the stress hormone cortisol. This increase is reflected in subsequent estrogen and progesterone production, which again can explain a late period and the presence of nausea.
Thyroid hormone alterations
too much (hyperthyroidism) or too little (hypothyroidism) thyroid hormones results in problems in achieving or carrying a pregnancy to term, miscarriages, pregnancy problems or premature delivery. In addition, these alterations are associated with anovulation and abnormal menstrual cycles.
the intake of certain medications can cause amenorrhea (absence of menstruation). Some examples could be antipsychotics, antidepressants and incorrect use of oral contraceptives.
Assisted reproduction treatments
in these patients, the use of vaginal progesterone sometimes delays menstrual bleeding. In addition, it can cause a delay in bowel emptying, resulting in nausea and emotional changes that are often mistaken for pregnancy symptoms.

Assisted procreation, as any other medical treatment, requires that you rely on the professionalism of the doctors and staff of the clinic you choose. Obviously, each clinic is different. Get now your Fertility Report, which will select several clinics for you out of the pool of clinics that meet our strict quality criteria. Moreover, it will offer you a comparison between the fees and conditions each clinic offers in order for you to make a well informed choice.

As we have discussed, there are many causes that can lead to a late period, not just pregnancy. Therefore, if you have any doubts, please don´t hesitate to contact a specialist

FAQs from users

Can PCOS give a false positive pregnancy test?

By Jana Bechthold M.D. (gynaecologist).

Pregnancy tests determine the presence of the hormone (beta-hCG= human beta chorionic gonadotropin) in the urine. This is produced when pregnancy occurs. A positive test means that this hormone is present in the urine and therefore the woman is pregnant.

Polycystic ovary syndrome (PCOS) is when the ovaries produce higher than normal amounts of androgens and this can interfere with the development and release of the egg. Sometimes, instead of eggs forming and maturing, cysts develop in the ovaries. Instead of the egg being released during ovulation, as it is in a normal menstrual cycle, the cysts grow and increase in size.

Only in the case of a cyst that produces hCG, which is very rare, the hormone would show in your urine and give a false-positive result with the pregnancy test.

Can a pregnancy test fail?

By Sara Salgado B.Sc., M.Sc. (embryologist).

Yes, the negative result can be wrong. The main reason is usually that the pregnancy test has been done too soon, but, as indicated in the article, it can happen because the test is in poor condition, because it is not highly sensitive or because there has been an early miscarriage, among other things.

I have symptoms of pregnancy after IVF, but the test was negative, what can this be due to?

By Juan José Espinós Gómez M.D., Ph.D. (gynecologist).

The symptoms of pregnancy in its early stages are totally unspecific and are often very similar to those that occur in premenstrual syndrome. Mood swings, headaches, abdominal distension, pain in the ovaries or feeling as if a period is coming, are common to both situations and are favored by the hormonal environment in the final phases of the menstrual cycle.

In some cases there is also a psychogenic component that is impossible to establish, that can have an influence to a greater or lesser extent depending on the individual.

I think I'm pregnant but the test is negative, what's wrong with me?

By Sara Salgado B.Sc., M.Sc. (embryologist).

Don't worry, everything is fine with you. Just repeat the test in about a week to confirm the result. If it's still negative, it's very likely that the symptoms you notice are due to another reason, not a pregnancy. If you are in doubt, it is best to contact your doctor for an ultrasound and an exploration, although if the pregnancy is very early you will not be able to see anything on the ultrasound and you should wait a few weeks.

How do I know if I'm pregnant if my test shows negative?

By Sara Salgado B.Sc., M.Sc. (embryologist).

The first thing would be to repeat the pregnancy test after about 5 days. If the test continues to be negative, it is more likely that your period will come soon, but if it does not, it is best to consult your doctor.

Is the result of a negative urine pregnancy test before the delay in menstruation reliable?

By Silvia Azaña Gutiérrez B.Sc., M.Sc. (embryologist).

It depends on the sensitivity of the test, i.e. the minimum amount of hCG hormone it can detect. However, for the result to be reliable, it's recommended to perform the pregnancy test once there is a missed period. This means that you should wait to take the test until there is a delay in your period with respect to the day you expected it to come. In this way, false negatives are avoided because the hormone should already be at levels detectable by the test.

Therefore, in this situation, we recommend repeating the test on the appropriate days to obtain a more reliable result.

If you want to know more about pregnancy symptoms, we recommend you visit this link: What Are the Most Common Symptoms of Pregnancy?

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Azizi M, Elyasi F. Biopsychosocial view to pseudocyesis: A narrative review. Int J Reprod Biomed. 2017 Sep;15(9):535-542.

Cole LA. The hCG assay or pregnancy test. Clin Chem Lab Med. 2012 Apr;50(4):617-30.

Dabadghao P. Polycystic ovary syndrome in adolescents. Best Pract Res Clin Endocrinol Metab. 2019 Jun;33(3):101272.

Deligeoroglou E, Creatsas G. Menstrual disorders. Endocr Dev. 2012;22:160-170.

Foster C, Al-Zubeidi H. Menstrual Irregularities. Pediatr Ann. 2018 Jan 1;47(1):e23-e28.

Griffey RT, Trent CJ, Bavolek RA, Keeperman JB, Sampson C, Poirier RF. "Hook-like effect" causes false-negative point-of-care urine pregnancy testing in emergency patients. J Emerg Med. 2013 Jan;44(1):155-60.

Montagnana M, Trenti T, Aloe R, Cervellin G, Lippi G. Human chorionic gonadotropin in pregnancy diagnostics. Clin Chim Acta. 2011 Aug 17;412(17-18):1515-20.

Tarín JJ, Hermenegildo C, García-Pérez MA, Cano A. Endocrinology and physiology of pseudocyesis. Reprod Biol Endocrinol. 2013 May 14;11:39.

Theofanakis C, Drakakis P, Besharat A, Loutradis D. Human Chorionic Gonadotropin: The Pregnancy Hormone and More. Int J Mol Sci. 2017 May 14;18(5):1059.

Upadhyay S. Pseudocyesis. JNMA J Nepal Med Assoc. 2008 Jul-Sep;47(171):147-50.

FAQs from users: 'Can PCOS give a false positive pregnancy test?', 'Can a pregnancy test fail?', 'I have symptoms of pregnancy after IVF, but the test was negative, what can this be due to?', 'I think I'm pregnant but the test is negative, what's wrong with me?', 'How do I know if I'm pregnant if my test shows negative?' and 'Is the result of a negative urine pregnancy test before the delay in menstruation reliable?'.

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Authors and contributors

 Ana Mª Villaquirán Villalba
Ana Mª Villaquirán Villalba
M.D., M.Sc.
Bachelor's Degree in Medicine from the University of Valle, Colombia. Specialist in Obstetrics & Gynecology. Master's Degree in Human Reproduction from the University of Valencia and IVI. Currently, she is the medical director of Tahe Fertilidad. More information about Ana Mª Villaquirán Villalba
License: 303007571
 Clara Miret Lucio
Clara Miret Lucio
B.Sc., M.Sc.
Bachelor's Degree in Pharmacy from the University of Valencia (UV). Master's Degree in Biotechnology of Human Assisted Reproduction from the UV. Experience as a pharmacist and embryologist specialized in Reproductive Medicine at Valencian Infertility Institute (IVI), and currently at Equipo Juana Crespo (Valencia, Spain). More information about Clara Miret Lucio
 Juan José  Espinós Gómez
Juan José Espinós Gómez
M.D., Ph.D.
Dr. Juan José Espinós Gómez has a degree in Medicine from the Central University of Barcelona - Reus - and has been awarded the Outstanding Graduate Award. More information about Juan José Espinós Gómez
Licence number: 080823651
 Sara Salgado
Sara Salgado
B.Sc., M.Sc.
Degree in Biochemistry and Molecular Biology from the University of the Basque Country (UPV/EHU). Master's Degree in Human Assisted Reproduction from the Complutense University of Madrid (UCM). Certificate of University Expert in Genetic Diagnosis Techniques from the University of Valencia (UV). More information about Sara Salgado
Adapted into english by:
 Cristina  Algarra Goosman
Cristina Algarra Goosman
B.Sc., M.Sc.
Graduated in Psychology by the University of Valencia (UV) and specialized in Clinical Psychology by the European University Center and specific training in Infertility: Legal, Medical and Psychosocial Aspects by University of Valencia (UV) and ADEIT.
More information about Cristina Algarra Goosman
Member number: CV16874
 Michelle Lorraine Embleton
Michelle Lorraine Embleton
B.Sc. Ph.D.
PhD in Biochemistry, University of Bristol, UK, specialising in DNA : protein intereactions. BSc honours degree in Molecular Biology, Univerisity of Bristol. Translation and editing of scientific and medical literature.
More information about Michelle Lorraine Embleton

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